Intrauterine Infusion of TGF-β1 Prior to Insemination, Alike Seminal Plasma, Influences Endometrial Cytokine Responses but Does Not Impact the Timing of the Progression of Pre-Implantation Pig Embryo Development

Martinez, Cristina A. and Cambra, Josep M. and Lucas, Xiomara and Ferreira-Dias, Graça and Rodriguez-Martinez, Heriberto and Gil, Maria A. and Martinez, Emilio A. and Cuello, Cristina and Parrilla, Inmaculada (2021) Intrauterine Infusion of TGF-β1 Prior to Insemination, Alike Seminal Plasma, Influences Endometrial Cytokine Responses but Does Not Impact the Timing of the Progression of Pre-Implantation Pig Embryo Development. Biology, 10 (2). p. 159. ISSN 2079-7737

[thumbnail of biology-10-00159.pdf] Text
biology-10-00159.pdf - Published Version

Download (1MB)

Abstract

Seminal plasma (SP) in the female genital tract induces changes that affect multiple reproductive processes. One of the active components in SP is the transforming growth factor β1 (TGF-β1), which has major roles in embryo development and pregnancy. Embryo transfer (ET) technology is welcomed by the pig industry provided that embryo quality at embryo collection as well as the fertility and prolificacy of the recipients after the ET is increased. This study evaluated different intrauterine infusion treatments at estrus (40 mL of SP, TGF-β1 cytokine in the extender, or the extender alone (control)) by mimicking an ET scenario in so-called “donor” (inseminated) and “recipient” (uninseminated) sows. On day 6 (day 0—onset of estrus), all “donors” were laparotomized to determine their pregnancy status (presence and developmental stage of the embryos). In addition, endometrial explants were collected from pregnant “donors” and cyclic “recipients,” incubated for 24 h, and analyzed for cytokine production. SP infusions (unlike TGF-β1 infusions) positively influenced the developmental stage of day 6 embryos. Infusion treatments differentially influenced the endometrial cytokine production, mainly in donors. We concluded that SP infusions prior to AI not only impacted the porcine preimplantation embryo development but also influenced the endometrial cytokine production six days after treatment, both in donors and recipients.

Item Type: Article
Subjects: e-Archives > Biological Science
Depositing User: Managing Editor
Date Deposited: 07 Jul 2023 04:05
Last Modified: 29 Jul 2025 03:50
URI: http://studies.sendtopublish.com/id/eprint/446

Actions (login required)

View Item
View Item